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KMID : 1094720180230010011
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Biotechnology and Bioprocess Engineering
2018 Volume.23 No. 1 p.11 ~ p.15
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R5 Peptide-based Biosilicification Using Methyltrimethoxysilane
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Park Jeong-Chan
Kim Do-Hyeon
Kim Chang-Sup
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Abstract
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We examined the performance of methyltrimethoxysilane (MTMS), a precursor of silicic acid, in the process of biosilicification induced by the R5 peptide from Cylindrotheca fusiformis. Recombinant GFP-R5 fusion protein was produced by Escherichia coli cultured at 25°C as a soluble and functional formation, but not at 37°C. MTMS-based biosilica deposits had a larger average diameter compared to tetraethyl orthosilicate (TEOS)-based deposits. Reducing phosphate concentration in the buffer system led to a decrease in the size of MTMS-based biosilica. These results provide insight into the surface modification of biosilica, and control of biosilica particle size, when using hydrophobic precursors such as MTMS.
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KEYWORD
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methyltrimethoxysilane, biosilica, hydrophobic silane, GFP-R5 fusion system
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